Alan

You could also take a look at this http://www.wlcastleman.com/equip/reviews/40D/livemicro/micro.htm

I know it uses a 40D and some seriously expensive microscope objectives but the results seem to be what you are aiming for. I can only dream of such a set up. I use a Canon G2 which I bought ...

Alan

In the end I found it was much better to use jpeg rather than raw. Try a stack of about 5 images to see the difference. I have been only really successfull using low powered i.e 3X microscope objective but I know others have success at higher powers.

The lighting and focus issues are common ...

I tried to post an image without success.

When I read the FAQ this seems impossible unless you have your own website which I haven't.

Thanks

Jon

Hi

This is the image

Hello can anyone help with what I am sure is a beginners lack of understanding.

I am using the trial version of Focus for series of images of spirogyra taken at 100X magnification with bright field illumination.

I am using a Canon G2 camera connected to a Wild M20 and can achieve good focused ...