Dear Stas,
thank you very much for your kind answer!
I have just upload the images into a folder named "microscopist - panorama.tif_files". These images are take with the ZEN software from Zeiss controlling a motorized microscope. Do you need more details from me? In any case I am curious to see ...

Dear All,

I experience difficulties with panorama stitching of microscopic images. I have made sure that I have enough overlap in the individual images, but still there are errors in stiching. What more parameters than overlap I have the possibility to optimize with helicon?

Thank you very much ...