Thanks for the workaround, it appears to be working. I have a new problem now though. When I enque 100 folders I never get 100 stacked images back. The number varies from 96 to 99, but never 100. For my timelapse to be accurate, I can't have missing frames like this. Do you have any idea as to why ...

I have a few hundred folders that each represent a single timepoint in a timelapse of an embryo during development. Each time point folder contains about ten different jpegs, each representing a different focal plane. See attachment 1. The first problem is that when I enque all of these folders, I ...