Posted: 26.12.2007 09:34
Danylo,
The sensor's physical size, and the pixels per side are specific values that are not prone to error. (Although with some cameras it may take some "Googling" to get the values for the active sensor area).
I like the method used by AutoMontage and some of the other "microscope" oriented software I've tried. But realistically, quite a few "non-professional" users of microscopes do not have access to a good stage micrometer.
Magnification values can be as accurate as the user wishes. Some absolutely need highly accurate magnification values and calibrate their systems accordingly through testing with a stage micrometer. Others will rely on the values engraved on their lenses or, with a microscope, use the product of the objectives and the relay optics as the recorded magnification.
Since I mostly use a compound microscope there are only 5 or 6 magnifications that I use regularly so it's pretty easy to have a sheet with those pixel values (or percentages) close at hand.
It just seems that whatever method is used for calculation or calibration, it should not be too difficult to make the process a bit more "automatic" in the Helicon Focus program.
Charlie
The sensor's physical size, and the pixels per side are specific values that are not prone to error. (Although with some cameras it may take some "Googling" to get the values for the active sensor area).
I like the method used by AutoMontage and some of the other "microscope" oriented software I've tried. But realistically, quite a few "non-professional" users of microscopes do not have access to a good stage micrometer.
Magnification values can be as accurate as the user wishes. Some absolutely need highly accurate magnification values and calibrate their systems accordingly through testing with a stage micrometer. Others will rely on the values engraved on their lenses or, with a microscope, use the product of the objectives and the relay optics as the recorded magnification.
Since I mostly use a compound microscope there are only 5 or 6 magnifications that I use regularly so it's pretty easy to have a sheet with those pixel values (or percentages) close at hand.
It just seems that whatever method is used for calculation or calibration, it should not be too difficult to make the process a bit more "automatic" in the Helicon Focus program.
Charlie