Sharpness parameter?

Optical microscopes + digital photography
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vaster04
Posts: 4
Joined: 14.10.2019 17:53

Sharpness parameter?

Post by vaster04 » 06.01.2020 17:35

If I understand principle of Helicon Focus operation correctly, method to determine what detail of stack image is in focus based on sharpness of this detail: if sharpness of detail higher than some threshold, it means that it is in focus, so it should be related to stack distance of this image.

If this is correct, what parameter of Helicon Focus interface is control level of sharpness threshold? Is it possible to change it?

Question is related to situation, when different microscope lenses have different aberration level, so threshold level should be different for different lenses.

Thank you

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Catherine
Posts: 405
Joined: 29.04.2019 22:38

Re: Sharpness parameter?

Post by Catherine » 15.01.2020 23:11

There is no absolute threshold at all, only the relative sharpness of two images is compared, there is no threshold expressed in absolute values.
On top of that, there is no direct control of this relative threshold either. Instead, there are Radius and Smoothing parameters.

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