Sharpness parameter?
Posted: 06.01.2020 17:35
If I understand principle of Helicon Focus operation correctly, method to determine what detail of stack image is in focus based on sharpness of this detail: if sharpness of detail higher than some threshold, it means that it is in focus, so it should be related to stack distance of this image.
If this is correct, what parameter of Helicon Focus interface is control level of sharpness threshold? Is it possible to change it?
Question is related to situation, when different microscope lenses have different aberration level, so threshold level should be different for different lenses.
Thank you
If this is correct, what parameter of Helicon Focus interface is control level of sharpness threshold? Is it possible to change it?
Question is related to situation, when different microscope lenses have different aberration level, so threshold level should be different for different lenses.
Thank you