Best stacking method for protozoa

Optical microscopes + digital photography
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MikeCT
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Joined: 08.04.2014 19:56

Best stacking method for protozoa

Post by MikeCT »

I am photography protozoa, which are basically transparent, and the resultant stacks are not as clear as I would like. Do any of you have experience with this, and do you haste any advice?

Many thanks
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Stas Yatsenko
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Re: Best stacking method for protozoa

Post by Stas Yatsenko »

I can only suggest to use method C, as it should deal with semitransparent structures better than other methods.
MikeCT
Posts: 116
Joined: 08.04.2014 19:56

Re: Best stacking method for protozoa

Post by MikeCT »

Thanks: interestingly each of the three methods is better in some parts of the image than others. However, Method C does seem to be the overall winner.
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Stas Yatsenko
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Re: Best stacking method for protozoa

Post by Stas Yatsenko »

Thanks. You can process the stack with different methods and then combine results on the Retouching tab. New version allows you to use alternative results as a source for cloning.
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