Stacked images that are slightly shifted

Optical microscopes + digital photography
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thescy
Posts: 1
Joined: 30.09.2015 18:08

Stacked images that are slightly shifted

Post by thescy »

I am trying to combine some images taken through the camera on my microscope. The issue I am having is that when I adjust the focus on my microscope, the image shifts slightly. As a result the object I am photographing moves very slightly from image to image. When I try to put them all together in Helicon Focus so that I can get a good image with everything in focus, that shift is messing up the program and I am getting a smeared looking image. Any suggestions on how to correct this? Is there some setting I need to adjust?
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Stas Yatsenko
Posts: 3841
Joined: 06.05.2009 14:05
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Re: Stacked images that are slightly shifted

Post by Stas Yatsenko »

Helicon Focus automatically aligns images but in predefined limits. I would suggest to increase limits for horizontal alignment in the Preferences.
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